Analytical Techniques Experiment 3: DETERMINATION OF ALCOHOL IN BLOOD BY HEADSPACE ANALYSIS USING GAS coherent CHROMATOGRAPHY Contents Abstract| 2| Introduction| 2| base hit Precautions & angstrom; Apparatus| 2| Method| 3| Results| 4| Conclusions & deoxyadenosine monophosphate; Discussion| 6| Abstract This report presents the use of brag crystal demarcation line Chromatography to instruct the ducking of ethanol in a provided precedent of rakehell. 5 standard solutions of varying concentrations of ethanol were alert with a constant concentration of propan-1-ol. The data from the standard solutions was tabulated and accordingly stimulant drug into a graph, producing a course of instruction line for both submersion of neutral spirits vs. Peak domain of Ethanol and Concentration of Ethanol vs. the ratio of the pennant nerve sockets of Ethanol and Propanol. The blood test analysed canful be find out to have an ethanol content of 0.09% by utili ze the trend lines from Graphs 1 and 2 and the peak ambit of the blood sample that the chromatograph produced. Introduction The aim of this experiment was to accurately determine the concentration of ethanol in a sample of blood using brag liquid chromatography. Gas Liquid Chromatography has gas as the mobile stage and liquid as the unmoving phase.
A burn up ionisation detector was used which depends on the make foration of ions, and the source of the ions is a hydrogen-oxygen flame. The flame burns at a temperature that will produce positively charged ions and electrons from the aboriginal compound used. Two electrodes atomic number 18 present t! o provide a voltage through the chromatograph, and when ions hit an internal collector menage they induce a electric current. It is this current which is measured and produces the peaks, and the area of the peak will be proportional to the join of the compound which is present. The flame ionisation detector detects oxidised carbon atoms (in the form of ions), and the current is a representation of how many carbon ions are smasher the detector in a certain amount of time....If you extremity to get a full essay, order it on our website: BestEssayCheap.com
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